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We present amulti-modal fiber array snapshot technique (M-FAST)based on an array of 96 compact cameras placed behind a primary objective lens and a fiber bundle array. Our technique is capable of large-area, high-resolution, multi-channel video acquisition. The proposed design provides two key improvements to prior cascaded imaging system approaches: a novel optical arrangement that accommodates the use of planar camera arrays, and a new ability to acquire multi-modal image data acquisition. M-FAST is a multi-modal, scalable imaging system that can acquire snapshot dual-channel fluorescence images as well as differential phase contrast measurements over a large 6.59 mm × 9.74 mm field-of-view at 2.2-μm center full-pitch resolution.

This paper experimentally examines different configurations of a multi-camera array microscope (MCAM) imaging technology. The MCAM is based upon a densely packed array of “micro-cameras” to jointly image across a large field-of-view (FOV) at high resolution. Each micro-camera within the array images a unique area of a sample of interest, and then all acquired data with 54 micro-cameras are digitally combined into composite frames, whose total pixel counts significantly exceed the pixel counts of standard microscope systems. We present results from three unique MCAM configurations for different use cases. First, we demonstrate a configuration that simultaneously images and estimates the 3D object depth across a 100×135mm²FOV at approximately 20 µm resolution, which results in 0.15 gigapixels (GP) per snapshot. Second, we demonstrate an MCAM configuration that records video across a continuous 83×123mm²FOV with twofold increased resolution (0.48 GP per frame). Finally, we report a third high-resolution configuration (2 µm resolution) that can rapidly produce 9.8 GP composites of large histopathology specimens.